| LSR

Lipolysis-stimulated lipoprotein receptor (LSR) as the host cell receptor for all members of the iota toxin family, including CDT. LSR is a type I, single-pass, transmembrane protein, featuring an Ig-like V-type domain in the extracellular part of the protein. The receptor is mainly expressed in the liver, but also in the intestine and various other tissues.LSR was identified as a hepatic receptor for triglyceride-rich lipoproteins . More recently, LSR act in the recruitment of tricellulin to tricellular contacts that are important for the integrity of epithelial barriers .
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.36	1.78	1.23	0.79	0.46	0.29	0.17	0.09
Corrected OD	2.27	1.69	1.14	0.7	0.37	0.2	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human LSR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-100	96
Cell Lysates(n=8)	93-105	102
Cell Culture Supernates(n=8)	96-108	106

 

| Linearity

Samples were spiked with high concentrations of Human LSR and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108